abstract：:Full-length cDNA clones of RNA 2 of tobacco rattle virus (TRV) strain PLB have been cloned into the transcription vector pPM1. Products of in vitro transcription by Escherichia coli RNA polymerase, either capped or uncapped, were as infectious as native RNA 2 when coinoculated with RNA 1 of TRV strain TCM. At least 70...

journal_title：Virology

authors： Angenent GC,Posthumus E,Bol JF

更新日期：1989-11-01 00:00:00

abstract：:Tomato strain L of tobacco mosaic virus (TMV-L) induces a hypersensitive response (necrotic local lesions) on tobacco plants with the N' gene. A factor responsible for induction of the hypersensitive response has been mapped to the coat protein gene. We have constructed several mutants which have insertions or deletio...

journal_title：Virology

authors： Saito T,Yamanaka K,Watanabe Y,Takamatsu N,Meshi T,Okada Y

更新日期：1989-11-01 00:00:00

abstract：:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...

journal_title：Virology

authors： Kaur P,McDougall JK

更新日期：1989-11-01 00:00:00

abstract：:Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...

journal_title：Virology

authors： Hyatt AD,Eaton BT,Brookes SM

更新日期：1989-11-01 00:00:00

abstract：:Gibbon ape leukemia virus (GaLV) is a highly oncogenic C-type retrovirus capable of inducing myeloid leukemia in juvenile gibbons. GaLV is antigenically most closely related to a new world monkey virus, simian sarcoma associated virus (SSAV), and less to the murine and feline C-type leukemia viruses. To begin to under...

journal_title：Virology

authors： Delassus S,Sonigo P,Wain-Hobson S

更新日期：1989-11-01 00:00:00

abstract：:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...

journal_title：Virology

authors： Pugh JC,Summers JW

更新日期：1989-10-01 00:00:00

abstract：:Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype we...

journal_title：Virology

authors： Trent DW,Grant JA,Monath TP,Manske CL,Corina M,Fox GE

更新日期：1989-10-01 00:00:00

abstract：:Vaccinia virus gene D12L, which lies between nucleotides 14,350 and 13,487 in the HindIII D fragment, is transcribed at early times in infection and is capable of encoding a protein 287 amino acids in length with a predicted molecular mass of 33,331. A polyclonal antiserum was raised in rabbits to a fusion protein con...

journal_title：Virology

authors： Niles EG,Lee-Chen GJ,Shuman S,Moss B,Broyles SS

更新日期：1989-10-01 00:00:00

abstract：:We have applied the procedure of Agrobacterium-mediated inoculation to develop a simple, efficient, and reproducible assay for the infectivity of the leafhopper-transmitted geminivirus, beet curly top virus (BCTV). This assay system was used to show that a coat protein mutant of BCTV is not infectious, but could be co...

journal_title：Virology

authors： Briddon RW,Watts J,Markham PG,Stanley J

更新日期：1989-10-01 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...

journal_title：Virology

authors： Pereira L,Ali M,Kousoulas K,Huo B,Banks T

更新日期：1989-09-01 00:00:00

abstract：:We have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (VSV) proteins and database entries. Unexpectedly, we found that the L polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similar...

journal_title：Virology

authors： McClure MA,Perrault J

更新日期：1989-09-01 00:00:00

abstract：:Human rotaviruses (Wa, KUN, MO) showed hemagglutination (HA) only with fixed 1-day-old chicken erythrocytes, and their HA activities were completely destroyed by trypsin activation of virions. Simian SA-11 and bovine NCDV had HA activities not only against fixed erythrocytes but also against fresh erythrocytes from va...

journal_title：Virology

authors： Fukudome K,Yoshie O,Konno T

更新日期：1989-09-01 00:00:00

abstract：:Fixed rabies virus strains (ERA and CVS) produce a fatal paralytic disease in mice after intracerebral or intramuscular injection. Some antigenic mutants of both CVS and ERA viruses with a substitution in position 333 of the glycoprotein (arginine is replaced either by a glutamine, a glycine, or an isoleucine) are tot...

journal_title：Virology

authors： Tuffereau C,Leblois H,Bénéjean J,Coulon P,Lafay F,Flamand A

更新日期：1989-09-01 00:00:00

abstract：:Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...

journal_title：Virology

authors： Quillet L,Guilley H,Jonard G,Richards K

更新日期：1989-09-01 00:00:00

abstract：:The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...

journal_title：Virology

authors： Mautner V,Mackay N,Steinthorsdottir V

更新日期：1989-08-01 00:00:00

abstract：:Foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability. Like other RNA viruses, this virus has a high rate of mutation. It has been proposed that selection exerted by the host's antibodies could play a major role in the rapid evolution of FMDV. The present work reports the selection of FMDV antib...

journal_title：Virology

authors： Carrillo EC,Rojas ER,Cavallaro L,Schiappacassi M,Campos R

更新日期：1989-08-01 00:00:00

abstract：:Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...

journal_title：Virology

authors： Huisman MJ,Cornelissen BJ,Groenendijk CF,Bol JF,van Vloten-Doting L

更新日期：1989-08-01 00:00:00

abstract：:The nonstructural rotavirus receptor glycoprotein NS28 is 175 amino acids long and oriented in the RER membrane with the NH2 terminus on the luminal side and approximately 131 amino acids accessible from the cytoplasmic side. Au et al. (1988) have demonstrated that NS28 is able to interact with rotavirus single-shelle...

journal_title：Virology

authors： Meyer JC,Bergmann CC,Bellamy AR

更新日期：1989-07-01 00:00:00

abstract：:When recombinant plasmids containing the entire adeno-associated virus (AAV) genome are transfected into permissive cells infected with a helper adenovirus, infectious AAV particles are efficiently generated. These plasmids can be used to generate mutant AAV genomes or recombinant AAV vectors. Packaging of mutant AAV ...

journal_title：Virology

authors： Chejanovsky N,Carter BJ

更新日期：1989-07-01 00:00:00

abstract：:A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 ...

journal_title：Virology

authors： Ritchie BW,Niagro FD,Lukert PD,Steffens WL 3rd,Latimer KS

更新日期：1989-07-01 00:00:00

abstract：:A recombinant vaccinia virus that expresses the nucleoprotein gene of Lassa virus (Josiah strain) under the control of the P7.5 promoter was constructed using the lacZ coexpression transfer vector pSC11. Southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene o...

journal_title：Virology

authors： Morrison HG,Bauer SP,Lange JV,Esposito JJ,McCormick JB,Auperin DD

更新日期：1989-07-01 00:00:00

abstract：:A range of deletion and other mutants in the coding region of the E1A gene of Ad5 has been assayed for transformation of baby rat kidney (BRK) cells in cooperation with ras, repression of the SV40 enhancer, and induction of proliferating cell nuclear antigen (PCNA). Transformation efficiency was drastically reduced by...

journal_title：Virology

authors： Jelsma TN,Howe JA,Mymryk JS,Evelegh CM,Cunniff NF,Bayley ST

更新日期：1989-07-01 00:00:00

abstract：:In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...

journal_title：Virology

authors： Winkler G,Maxwell SE,Ruemmler C,Stollar V

更新日期：1989-07-01 00:00:00

abstract：:GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...

journal_title：Virology

authors： Koltunow AM,Rezaian MA

更新日期：1989-06-01 00:00:00

abstract：:An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...

journal_title：Virology

authors： Tanese N,Roth M,Epstein H,Goff SP

更新日期：1989-06-01 00:00:00

abstract：:Analyses of reassortant and parental strains of BTV serotypes 3 and 10, in serum neutralization tests, confirmed the major role of outer capsid protein VP2 in determination of virus serotype and its involvement in serum neutralization. However, a reassortant BTV strain (R70), containing protein VP5 derived from BTV 3 ...

journal_title：Virology

authors： Mertens PP,Pedley S,Cowley J,Burroughs JN,Corteyn AH,Jeggo MH,Jennings DM,Gorman BM

更新日期：1989-06-01 00:00:00

abstract：:Genomic and messenger RNAs of a Zaire strain of Ebola virus were cloned, and inserts specific for the nucleoprotein gene were isolated and sequenced. The nucleoprotein gene is located proximal to the 3' end of the genome and is preceeded by a putative leader sequence. The gene begins with the transcriptional start sit...

journal_title：Virology

authors： Sanchez A,Kiley MP,Holloway BP,McCormick JB,Auperin DD

更新日期：1989-05-01 00:00:00

abstract：:Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...

journal_title：Virology

authors： Heaton LA,Carrington JC,Morris TJ

更新日期：1989-05-01 00:00:00

abstract：:High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot ...

journal_title：Virology

authors： Luckow VA,Summers MD

更新日期：1989-05-01 00:00:00

abstract：:Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...

journal_title：Virology

authors： Sato H,Watanabe S,Furuno A,Yoshiike K

更新日期：1989-05-01 00:00:00

abstract：:We have quantitated replication of the RNA genomes of vesicular stomatitis virus (VSV) and its defective interfering (DI) particles in a BHK21 cell-free system into which nucleocapsids were introduced in varying amounts and ratios, with or without addition of purified virus polymerase components. The quantitative tran...

journal_title：Virology

authors： Giachetti C,Holland JJ

更新日期：1989-05-01 00:00:00

abstract：:This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...

journal_title：Virology

authors： Miura T,Shibuya M,Tsujimoto H,Fukasawa M,Hayami M

更新日期：1989-04-01 00:00:00

abstract：:Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with S...

journal_title：Virology

authors： Boggs WM,Hahn CS,Strauss EG,Strauss JH,Griffin DE

更新日期：1989-04-01 00:00:00

abstract：:An antiserum was raised against a synthetic peptide corresponding to the 18 C-terminal amino acids of a putative 16K protein encoded by the 3'-terminal open reading frame of tobacco rattle virus (TRV) RNA-1. This antiserum was used to demonstrate expression of the 16K cistron in vivo. TRV-infected tobacco protoplasts ...

journal_title：Virology

authors： Angenent GC,Verbeek HB,Bol JF

更新日期：1989-04-01 00:00:00

abstract：:Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...

journal_title：Virology

authors： Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

更新日期：1989-03-01 00:00:00

abstract：:We have previously shown that the late promoter of the human papovavirus BK (prototype) is contained within the three 68-bp repeats and a 66-bp region to the late side of the repeats which together constitute the early promoter enhancer. We have now carried out unidirectional deletion and linker scan analyses of these...

journal_title：Virology

authors： Cassill JA,Deyerle KL,Subramani S

更新日期：1989-03-01 00:00:00

abstract：:We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....

journal_title：Virology

authors： Ogston CW,Schechter EM,Humes CA,Pranikoff MB

更新日期：1989-03-01 00:00:00

abstract：:Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the r...

journal_title：Virology

authors： Willingmann P,Barnert H,Zeichhardt H,Habermehl KO

更新日期：1989-02-01 00:00:00

abstract：:Herpes simplex virus mutant KG111 contains a nonsense mutation at codon 44 of the viral thymidine kinase (tk) gene and produces low amounts of a truncated tk polypeptide. We tested mutant KG111 and related viruses that specify varying amounts of similar truncated tk polypeptides for their sensitivities to antiviral nu...

journal_title：Virology

authors： Coen DM,Irmiere AF,Jacobson JG,Kerns KM

更新日期：1989-02-01 00:00:00

abstract：:TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...

journal_title：Virology

authors： Atreya CD,Siegel A

更新日期：1989-02-01 00:00:00